heliX +
Imaniwali yosebenzisayo

I-ADAPTER STRAND PACKAGE

ngodayi obomvu u-Ra
I-Dynamic Biosensors GmbH & Inc.
I-ASP-1-Ra v5.1

Izici Eziyinhloko

• I-Adapter strand 1 – Ra ne-Adapter strand 2 – Ra – lfs (ligand free strand) ukuze isebenze i-heliX® Adapter Chip ku-Spot 1 kanye ne-Spot 2, ngokulandelana.
• Ihambisana ne-heliX® Adapter Chip.
• Kufaka phakathi imicu ye-Adapter yokwenziwa kabusha okungu-50.
• Ilungele i-MIX&RUN sample ukulungiselela.
• Imicu ye-adaptha 1 kanye no-2 iphethe udayi obomvu we-hydrophilic (Ra) ene-net charge eyodwa.

I-heliX® Adapter Chip Overview

Izindawo ezi-2 ezinokulandelana kwesikhonkwane oku-2 okuhlukile kwekheli elinekhodi ye-DNA.

Incazelo Yomkhiqizo

Inombolo ye-oda: ASP-1-Ra
Ithebula 1. Okuqukethwe kanye Nokwaziswa Kwesitoreji

Okubalulekile	Cap	Ukugxila	Inani	Ibhafa	Isitoreji
I-adaptha umucu 1 – Ra	Mnyama	400 nM	5 x 100µL	I-TE40 [1]	-20°C
I-adaptha umucu 2 – Ra – lfs	Emhlophe	200/250 nM	5 x 200µL	I-TE40 [1]	-20°C

Okokusetshenziswa kocwaningo kuphela.
Lo mkhiqizo unomkhawulo wempilo yeshelufu, sicela ubone idethi yokuphelelwa yisikhathi kulebula.
Ukuze ugweme imijikelezo eminingi yokuncibilika kweqhwa sicela uliquot i-nanolever.

Ukulungiselela | MIX&RUN

I-in-solution hybridization ye-adaptha nemicu ye-ligand:

1. Hlanganisa umucu we-Adaptha 1 – Ra (400 nM) kanye ne-conjugated Ligand strand (500 nM) ngesilinganiso esingu-1:1 (v/v).
2. Faka isixazululo sesinyathelo 1 ku-RT ku-600 rpm imizuzu engama-30 ukuze uqinisekise ukuhlanganisa okuphelele.
3. Hlanganisa isisombululo sesinyathelo sesi-2 ne-Adapter strand 2 – Ra – lfs (200 nM) ngesilinganiso esingu-1:1 (v/v). Isixazululo sesilungele ukusetshenziswa ekusebenzeni kwe-biochip.
   Ukuzinza kwesixazululo kuhlobene nokuzinza kwe-molecule ye-ligand.

Ithebula 2. Izinto ezengeziwe zokusebenzisa indawo 1 nendawo eyinkomba 2.

Okubalulekile	Ukugxila	Ibhafa	Igama Lomkhiqizo Ohlobene	I-oda No
I-Ligand strand ethwele i-conjugated ligand	500 nM	I-PE40 [2]	I-heliX® Amine Coupling Kit 1	I-HK-NHS-1

Example

Ivolumu edingekayo yokusebenza oku-3: 100 µL nokugxiliswa kokugcina okungu-100 nM.

I-Vial 1		I-Vial 2
I-adaptha umucu 1 – Ra (400 nM)	I-Ligand strand ehlanganisiwe (500 nM)	I-adaptha umucu 2 – Ra – lfs (200/250 nM)
25µL	25µL	50µL

Ngemva kwesikhathi sokufukamela, hlanganisa ibhodlela 1 kanye nebhodlela 2 ukuze uthole u-100 µL wesixazululo se-DNA esilungele ukusetshenziswa.

Oxhumana naye

I-Dynamic Biosensors GmbH I-Perchtinger Str. 8/10 81379 eMunich EJalimane

Inkampani engu-Dynamic Biosensors, Inc. 300 Trade Center, Suite 1400 I-Woburn, i-MA 01801 USA

Oda Ulwazi order@dynamic-biosensors.com
Ukusekela kwezobuchwepheshe support@dynamic-biosensors.com

www.dynamic-biosensors.com

Izinsimbi nama-chips akhiwe futhi akhiqizwa eJalimane.
©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. Wonke amalungelo agodliwe.

[1] TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA
[2] Uma iphrotheni ingazinzile ku-PE40 (TE40, HE40),
sicela uhlole ukuhambisana kwebhafa neshidi lokuhambisana le-switchSENSE®.
I-ASP-1-Ra v5.1
www.dynamic-biosensors.com

Amadokhumenti / Izinsiza

i-BIOSENSORS ASP-1-Ra Adapter Strand Package [pdf] Imaniwali yosebenzisayo ASP-1-Ra, ASP-1-Ra v5.1, ASP-1-Ra Adapter Strand Package, ASP-1-Ra, Adapter Strand Package, Strand Package

Izithenjwa

• Imaniwali yosebenzisayo