I-BIOSENSORS heliX enamandla kanye ne-Adapter Strand 1

Imininingwane:

• Igama lomkhiqizo: heliX+
• Imodeli: I-ADAPTER STRAND 1 enodayi obomvu u-Ra
• Umkhiqizi: I-Dynamic Biosensors GmbH & Inc.
• Inombolo ye-oda: AS-1-Ra
• Ukugxila: 400 nM
• Isitoreji: Ikepisi elimnyama, iBuffer TE40
• Okokusetshenziswa kocwaningo kuphela

Incazelo Yomkhiqizo:

I-heliX+ ADAPTER STRAND 1 iklanywe ngodayi obomvu i-Ra futhi ifaka amachashazi angu-2 nama-2 ahlukene okulandelana kwezikhonkwane zokukhuluma nge-DNA-encoded. Iza ekuhlanganiseni okungu-400 nM futhi ihloselwe izinjongo zocwaningo.

Imiyalo yokusebenzisa:
Ukulungiselela | MIX&RUN:

1. Hlanganisa umucu we-Adapter 1 – Ra (400 nM) kanye ne-conjugated Ligand strand (500 nM) ngesilinganiso esingu-1:1 (v/v).
2. Faka isixazululo kusuka kusinyathelo 1 ekamelweni lokushisa ku-600 rpm imizuzu engu-30 ukuqinisekisa ukuxutshwa okuphelele.
3. Hlanganisa isixazululo esisuka kusinyathelo sesi-2 ne-Adapter strand 2 – Ra – lfs (200 nM) ngesilinganiso esingu-1:1 (v/v).
4. Isixazululo manje sesilungele ukusetshenziselwa ukusebenza kwe-biochip.

Qaphela: Ukuzinza kwesixazululo kuncike ekuzinzeni kwe-molecule ye-ligand.

I-FAQ:

1. Q: Ithini impilo yeshelufu ye-heliX+ ADAPTER STRAND 1?
   IMP: Umkhiqizo unomkhawulo wempilo yeshelufu, sicela ubheke idethi yokuphelelwa yisikhathi kulebula.
2. Q: Ingabe i-heliX+ ADAPTER STRAND 1 ingamiswa iqhwa?
   IMP: Ukuze ugweme imijikelezo eminingi yokuqandisa, kuyanconywa ukuthi usebenzise i-nanolever.

heliX+
Imaniwali yosebenzisayo

I-ADAPTER STRAND 1
ngodayi obomvu Rb
I-Dynamic Biosensors GmbH & Inc.
AS-1-Rb v5.1

Izici Eziyinhloko

•  I-Adapter strand 1 – Rb ukuze isebenze i-heliX® Adapter Chip Spot 1.
• Ihambisana ne-heliX® Adapter Chip.
• Kufaka phakathi imicu ye-Adapter yokwenziwa kabusha okungu-50.
• Ilungele i-MIX&RUN sample ukulungiselela.
• I-adaptha umucu 1 iphethe udayi obomvu we-hydrophilic (Rb) onenani eliphakathi nendawo.

I-heliX® Adapter Chip Overview
Izindawo ezi-2 ezinokulandelana kwesikhonkwane oku-2 okuhlukile kwekheli elinekhodi ye-DNA.

Incazelo Yomkhiqizo

Inombolo ye-oda: AS-1-Rb
Ithebula 1. Okuqukethwe kanye Nokwaziswa Kwesitoreji

Okubalulekile	Cap	Ukugxila	Inani	Ibhafa	Isitoreji
I-adaptha umucu 1 – Rb	Mnyama	400 nM	5 x 100µL	I-TE40 [1]	-20°C

Okokusetshenziswa kocwaningo kuphela.

• Lo mkhiqizo unomkhawulo wempilo yeshelufu, sicela ubone idethi yokuphelelwa yisikhathi kulebula.
• Ukuze ugweme imijikelezo eminingi yokuncibilika kweqhwa sicela uliquot i-nanolever

Ukulungiselela | MIX&RUN

I-in-solution hybridization ye-adaptha nemicu ye-ligand:

1.  Hlanganisa umucu we-Adaptha 1 – Rb (400 nM) kanye ne-conjugated Ligand strand (500 nM) ngesilinganiso esingu-1:1 (v/v).
2.  Faka isixazululo sesinyathelo 1 ku-RT ku-600 rpm imizuzu engama-30 ukuze uqinisekise ukuhlanganisa okuphelele.
3.  Hlanganisa isisombululo sesinyathelo sesi-2 ne-Adapter strand 1 – Rb – lfs (200 nM) ngesilinganiso esingu-1:1 (v/v). Isixazululo sesilungele ukusetshenziswa ekusebenzeni kwe-biochip. Ukuzinza kwesixazululo kuhlobene nokuzinza kwe-molecule ye-ligand

Ithebula 2. Izinto ezengeziwe zokusebenzisa indawo 1 nendawo eyinkomba 2.

Okubalulekile	Ukugxila	Ibhafa	Igama Lomkhiqizo Ohlobene	I-oda No
I-Ligand umucu ethwele umsipha ohlangene	500 nM	P2] E40 [	indiza enophephela emhlaneX® Ikhithi yokuhlanganisa ye-Amine 1	I-HK-NHS-1
I-adaptha umucu 1 – Rb – lfs	200/250 nM	I-TE40 [1]	I-adapter strand 2 ngodayi obomvu Rb prehybridized nge-ligand-free strand	AS-2-Rb-lfs

Example
Ivolumu edingekayo yokusebenza oku-3: 100 μL nokugxiliswa kokugcina kwe-100 nM.

I-Vial 1		I-Vial 2
I-adaptha umucu 1 – Rb (400 nM)	Kuhlanganiswe Intambo ye-Ligand (500 nM)	I-adaptha umucu 1 – Rb – lfs (200/250 nM)
25µL	25µL	50µL

Ngemva kwesikhathi sokufukamela, hlanganisa ibhodlela 1 kanye nebhodlela 2 ukuze uthole u-100 µL wesixazululo se-DNA esilungele ukusetshenziswa.

Oxhumana naye
I-Dynamic Biosensors GmbH

• I-Perchtinger Str. 8/10
• 81379 eMunich
• EJalimane

Inkampani engu-Dynamic Biosensors, Inc.

• 300 Trade Center, Suite 1400
• I-Woburn, i-MA 01801
• USA

Oda Ulwazi order@dynamic-biosensors.com
Ukusekela kwezobuchwepheshe support@dynamic-biosensors.com

www.dynamic-biosensors.com
Izinsimbi nama-chips akhiwe futhi akhiqizwa eJalimane. ©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. Wonke amalungelo agodliwe.

1. TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA
2. Uma iphrotheni ingazinzile ku-PE40 (TE40, HE40), sicela uhlole ukuhambisana kwebhafa neshidi lokuhambelana le-switchSENSE®.

www.dynamic-biosensors.com

Amadokhumenti / Izinsiza

I-BIOSENSORS heliX enamandla kanye ne-Adapter Strand 1 [pdf] Imaniwali yosebenzisayo AS-1-Ra, AS-2-Ra-lfs, heliX plus Adapter Strand 1, heliX plus, Adapter Strand 1, Strand 1

Izithenjwa

• Imaniwali yosebenzisayo