Optimize cell immobilization performance with CELL SOLUTION 1 FOR heliX cyto (CLL-SOL-1) by Dynamic Biosensors GmbH. Ideal for single-cell Interaction Cytometry experiments with HeLa cells and labeled analytes. Follow usage instructions for best results.
Zitholele i-heliX cyto Uhlelo Lokuhlaziya Ilabhorethri Ngokugcwele Okuzenzakalelayo ngeDynamic Biosensors GmbH. Funda mayelana ne-single-cell Interaction Cytometry kanye nendlela yokukala i-kinetics ebophezelayo yama-molecule anelebuli ekhanyayo. Thola imiyalo ngokusetshenziswa kwe-chip, ukulungiswa, isigcinalwazi esisebenzayo, ukuhlaziya i-sensorgram, i-passivation, kanye nokujwayelekile. Ama-FAQ amboza ukusebenziseka kabusha kwe-chip kanye nenjongo ye-Maintenance Chip. Ingcweti yobuciko bokulinganisa okunembayo ngalolu hlelo olusha.
Le manuwali yomsebenzisi inikeza imiyalelo enemininingwane yokusebenzisa i-Y-Structure Amine Coupling Kit 1 (HK-NYS-NHS-1) ngama-Dynamic Biosensors. Ikhithi inika amandla ukuhlangana kwama-biomolecules ngama-amine ayinhloko engalweni ebomvu yesakhiwo sika-Y, enikeza ukuhamba komsebenzi okuzinyathelo ezi-3 zokuhlanganisa ngezikhathi zokucubungula okusheshayo. Izinto ezengeziwe ezidingekayo zenqubo zibaliwe, kanye nemihlahlandlela ebalulekile nezinyathelo zokuphepha zokuqinisekisa ukuhlangana okuphumelelayo. Uma kuphakama izinkinga, abasebenzisi bangafuna usizo ethimbeni losekelo.
Zitholele i-HK-NYS-1 Helix Amine Coupling Kit ukuze uthole ama-assay abophezelayo asondelene emkhakheni wocwaningo. Yakha i-Y-structures yokubopha kanambambili kanye ne-ternary ngemicu emisha nama-ligand. Funda indlela yokusetha ama-assays ku-heliOS ukuze ufunde ukwakheka okuyinkimbinkimbi nama-molecule e-bispecific. Thola imiyalelo yokusetshenziswa namathiphu okuxazulula inkinga kuleli bhukwana eliphelele.
Zitholele indlela yokulebula ngempumelelo abahlaziyi ngama-amine ayinhloko usebenzisa i-heliXcyto Lebeling Kit Green Dye (Imodeli: CY-LK-G1-1). Le manuwali yomsebenzisi inikeza imiyalelo yesinyathelo ngesinyathelo, izici ezibalulekile, namathiphu abalulekile okulebula amaprotheni alungile. Funda mayelana nesikhathi sokusebenza, ubude besikhathi sokufukamela, inqubo yokuhlanza, nokubala I-Dye-to-Protein Ratio. Khulisa ukusebenza kahle kokulebula ngaphansi kwamaminithi angu-45 ngale khithi ephelele evela ku-Dynamic Biosensors GmbH.
Funda konke mayelana nePhakheji ye-Adapter Strand ye-heliX+ ASP-1-Ga v5.1 evela ku-Dynamic Biosensors GmbH. Zitholele izici ezibalulekile, ukucaciswa komkhiqizo, nemiyalo yokusetshenziswa kokusebenza kahle kwe-biochip. Oda imicu yakho ye-adaptha ye-ASP-1-Ga namuhla!
Zitholele i-heliX cyto Normalization Solution (NOR-R2) - umkhiqizo osuselwe kudayi obomvu wokulinganisa okunembile kwe-scIC. Hlela amasignali e-fluorescent ngalesi sixazululo esibalulekile. Funda ngokulungiselela, uhlelo lokusebenza, kanye nezici ezibalulekile ukuze usebenze kahle.
Imanuwali yomsebenzisi ye-Dynamic Biosensors' HK-GFP-1 v3.1 GFP Capture Kit inikeza imininingwane enemininingwane nemiyalelo yokusetshenziswa ye-heliX + GFP Capture Kit enodayi obomvu u-Ra. Funda mayelana nezici ezibalulekile, incazelo yomkhiqizo, inqubo yokulinganisa, nokuningi. Ukuze uthole imininingwane egcwele yobuchwepheshe, bheka imanuwali yomkhiqizo esemthethweni.
Zitholele imanuwali yomsebenzisi ye-AS-2-Ra v5.1 Adapter Strand ye-Dynamic Biosensors GmbH & Inc. Funda mayelana nokucaciswa, izici ezibalulekile, nemihlahlandlela yokulungiselela lo mkhiqizo osebenzayo we-heliX+. Hlola izinzuzo ze-Adapter Strand 2 enodayi obomvu i-Ra ngokusebenza kwe-biochip kanye ne-MIX&RUN sample ukulungiselela.
Zitholele imininingwane nemiyalelo yokusetshenziswa ye-AS-1-Gc v5.1 Adapter Strand ku-heliX+ User Manual by Dynamic Biosensors. Funda mayelana nezici zayo ezibalulekile, izincomo zesitoreji, nokuthi ungasilungiselela kanjani isisombululo sokusebenza kwe-biochip. Thola mayelana nenombolo ye-oda ye-Adapter Strand 2 ehlanganiswe ngaphambili ne-ligand-free strand - HK-NHS-1 AS-2-Gb-lfs.
