Zitholele incwadi yomsebenzisi ye-heliX+ TS-0 v5.1 Test and Standby Solution. Funda ngezici ezibalulekile, ukucaciswa, imiyalelo yesitoreji, ukusetshenziswa komkhiqizo, kanye nemibuzo evame ukubuzwa. Qinisekisa ukugcinwa okufanele kanye nokusebenza kwe-biochip ukuze uthole imiphumela emihle.
Funda mayelana ne-BU-HE-40-10 v2.1 Running Buffer, umkhiqizo we-Dynamic Biosensors, odizayinelwe ukuhlolwa kwezinto eziphilayo. Landela imiyalelo yokusetshenziswa ukuze usebenze kahle nemihlahlandlela yesitoreji ukuze usebenze kahle. Thola ukwesekwa kobuchwepheshe nolwazi lwe-oda ngaphakathi kwebhukwana.
Zitholele indlela yokuhlanza nokugcina kahle i-heliXcyto yakho nama-chips nge-CS-100-1 HeliXcyto Discover Molecule Cell Cleaning Solution 1. Lesi sixazululo esisekelwe okokuhlanza siklanyelwe ngokuqondile izinqubo zokuhlanza zansuku zonke, eziqinisekisa ukusebenza kahle. Landela imiyalelo yokusetshenziswa enikezwe kumanuwali yomsebenzisi ukuze uthole imiphumela ehamba phambili.
Funda ukuthi ungayilungiselela kanjani kahle futhi usebenzise i-heliXcyto RT-IC Running Buffer 1 (RB 1) ene-concentration yesitoko esingu-10x kanye nenani elingu-50 mL. Qinisekisa ukusebenza okuphelele kokulungiswa kanye nezilinganiso ze-RT-IC kudivayisi ye-heliXcyto.
Zitholele indlela yokuhlanza nokugcina ngempumelelo ama-chips akho e-heliXcyto nge-CCK-1-1 Dynamic Biosensors User Manual. Funda iphrothokholi efanele usebenzisa Isixazululo Sokuhlanza 1 kanye Nempushana Ye-Enzyme ukuze uthole ukusebenza kahle kwe-chip. Ukuhlanza njalo kuqinisekisa imiphumela enembile.
Funda mayelana ne-PF-BU-B-5 Running Buffer yohlelo lwe-proFIRE kule manuwali yomsebenzisi. Thola imininingwane yomkhiqizo, imihlahlandlela yokusebenzisa, imiyalelo yesitoreji, nokuningi. Qinisekisa ukuphathwa ngendlela efanele kanye nokulahlwa kwebhafa ukuze usebenze kahle.
Thuthukisa inqubo yakho yokuhlanganisa amaprotheni-DNA nge-PF-BU-C-1 Conjugation Buffer ngama-Dynamic Biosensors. Zitholele imiyalelo yokusetshenziswa enemininingwane nolwazi lwesitoreji kubhukwana lomsebenzisi. Khulisa ukusebenza kahle kwezidingo zakho zocwaningo nge-proFIRE 1X Conjugation Buffer.
Gcina idivayisi yakho ye-heliXcyto isesimweni esikahle nge-heliXcyto Cleaning Solution 3. Landela iziqondiso ezinembile zokunakekelwa okuphumelelayo, ukuqinisekisa ukuphila isikhathi eside nokusebenza. Inombolo ye-oda: CS-50-3.
Funda ukuthi ungayenza kanjani i-DNA isebenze kahle enamaprotheni amakhulu kuno-5 KDA usebenzisa i-PF-SH-1 Thiol Coupling Kit 1 yamaphrotheni. Le manuwali yomsebenzisi ihlinzeka ngokuhamba komsebenzi okunezinyathelo ezi-3 ezinemininingwane kanye nezici ezibalulekile ezifana namaqembu asebenzayo we-thiol kanye nokuhlanzwa kwekholomu ye-spin. Thola mayelana nezinto ezinconyiwe kanye nezinyathelo zemiphumela emihle ekuhlanganiseni kwe-protein-DNA.
Zitholele ulwazi olujulile nge-heliX Plus Adapter Strand 1 ngaphandle kwe-Fluorophore kule manuwali yomsebenzisi. Funda mayelana ne-BIOSENSORS enamandla kanye nobuchwepheshe obusha ngemuva kwe-Strand 1 no-Fluorophore. Hlola amakhono nezici zalo mkhiqizo othuthukisiwe ngezidingo zakho zocwaningo.
