Zitholele indlela yokuthuthukisa izilinganiso ze-RT-IC Kusiteshi Esikuhlaza usebenzisa imanuwali yomsebenzisi ye-NOR-Ga heliXcyto Normalization Solution (Idayi Eluhlaza). Funda ngezici ezibalulekile, ukulungiselela, amanothi ohlelo lokusebenza, nolwazi lokuxhumana lwe-Dynamic Biosensors GmbH ne-Inc.
Thola ukuthi i-heliXcyto, isixazululo esijwayelekile (udayi obomvu) yi-Dynamic Biosensors GmbH & Inc. (Inombolo Ye-oda: NOR-Ra), ithuthukisa ukukalwa kwe-RT-IC esiteshini esibomvu ngezinjongo zocwaningo. Funda mayelana nokucaciswa kwayo, ukulungiswa, amanothi ohlelo lokusebenza, nemihlahlandlela yesitoreji.
Zitholele ukuthi ungayenza kanjani i-DNA isebenze kahle usebenzisa i-PF-AB-1 Antibody Oligo Conjugation Kit. Le manuwali yomsebenzisi inikeza inqubo enezinyathelo ezi-3 enemininingwane yokuguqulwa kwe-DNA, ukuhlanganisa amaprotheni, nokuhlanzwa, okuholela ekuhlanganiseni kwekhwalithi ephezulu ye-antibody-DNA. Funda mayelana nezinto ezidingekayo, umugqa wesikhathi, namanothi abalulekile wokuhlanganisa ngempumelelo.
I-PF-NH2-1 Amine Coupling Kit eyenziwe nge-BIOSENSORS v6.1 enamandla ilungele ukusebenza kwe-thiol-DNA ngama-amine wamaprotheni angaphezu kuka-5 kDa. Le khithi inikezela ngokugeleza komsebenzi okuzinyathelo ezi-3 zokuhlanganisa nezinto zokuxhumanisa amaprotheni-DNA asebenzayo ngaphansi kwamahora angu-3. Gwema ukusebenzisa izinciphisi ezisekelwe ku-thiol futhi ulandele imiyalelo enikeziwe ukuze uthole imiphumela eyimpumelelo.
Thuthukisa ukusebenza kwe-biochip nge-LFS-0 Helix Ligand Free Strand. Lo mucu ongenayo i-Ligand uhlinzeka ngezindawo ezi-2 zokulandelana kwehange okuhlukile kwekheli elifakwe ikhodi ye-DNA. Ilungele ukusetshenziswa kwe-in vitro, i-LFS-0 inikezela ngokuhlangana okungama-500 nM futhi iyahambisana ne-SwitchSENSE Adapter Biochips. Qinisekisa isitoreji esifanele ukuze welule impilo yeshalofu futhi uxhumane ne-support@dynamic-biosensors.com ukuze uthole usizo.
Imanuwali yomsebenzisi ye-HK-NHS-1 Helix Amine Coupling Kit inikeza imiyalelo enemininingwane yokusebenza kwe-Ligand strand ngama-biomolecules aqukethe ama-amine ayinhloko. Le khithi ivumela ukuhlanganisa okulula nokuphumelelayo kwama-ligand amaningi, okukhipha i-high-purity ligand-DNA conjugates. Ilungele ukusetshenziswa kwe-in vitro, ikhithi iyahambisana nama-chips ahlukahlukene nezindlela zokuhlanza, enikeza umkhiqizo wokugcina onqunywe ngumsebenzisi.
Zitholele i-PF-NH2-3 Amine Coupling Kit 3 yamanuwali yomsebenzisi wamaphrotheni, ihlinzeka ngemininingwane enemininingwane, izinyathelo zokuhamba komsebenzi, namanothi abalulekile okuhlanganiswa kwe-in-vitro okuphumelelayo. Funda ngezici ezibalulekile nezinto ezengeziwe ezidingekayo ukuze kuhlanganiswe ngempumelelo amaprotheni.
Incwadi yomsebenzisi ye-TS-0 HeliX Test and Standby Solution evela ku-Dynamic Biosensors inikeza imiyalelo enemininingwane yokusebenzisa ikhithi yomucu we-adaptha, okuhlanganisa ukugcinwa okubalulekile nolwazi lokusebenzisa. Lo mkhiqizo we-in vitro uklanyelwe ukusebenza kahle kwe-heliX Adapter Biochip Spot 1 kanye ne-Spot 2, enodayi obomvu u-Ra kanye nenetha eyodwa eshajwayo. Xhumana nama-Dynamic Biosensors ngemibuzo ehlobene ne-oda noma yosekelo lobuchwepheshe.
