I-BIOSENSORS heliX enamandla kanye ne-Adapter Strand 2

Izici Eziyinhloko

• I-Adapter strand 2 yokusebenza kwe-HeliX® Adapter Chip Spot 2.
• Ihambisana ne-heliX® Adapter Chip.
• Kufaka phakathi imicu ye-Adapter yokwenziwa kabusha okungu-50.
• Ilungele i-MIX&RUN sample ukulungiselela.
• I-Adapter strand 2 iphethe udayi obomvu we-hydrophobic (Rc) onokushaja okuphakathi nendawo.

I-heliX® Adapter Chip Overview

Izindawo ezi-2 ezinokulandelana kwesikhonkwane oku-2 okuhlukile kwekheli elinekhodi ye-DNA.

Incazelo Yomkhiqizo

Inombolo ye-oda: AS-2-Rc-lfs
Ithebula 1. Okuqukethwe kanye Nokwaziswa Kwesitoreji

Okubalulekile	Cap	Ukugxila	Inani	Ibhafa	Isitoreji
I-adaptha umucu 2 – Rc – lfs	Emhlophe	200/250 nM	5 x 200µL	I-TE40 [1]	-20°C

Okokusetshenziswa kocwaningo kuphela.
Lo mkhiqizo unomkhawulo wempilo yeshelufu, sicela ubone idethi yokuphelelwa yisikhathi kulebula.
Ukuze ugweme imijikelezo eminingi yokuncibilika kweqhwa sicela uliquot i-nanolever.

Ukulungiselela | MIX&RUN

I-in-solution hybridization ye-adaptha nemicu ye-ligand:

1. Hlanganisa umucu we-Adapter 1 – Rc (400 nM) kanye ne-conjugated Ligand strand (500 nM) ngesilinganiso esingu-1:1 (v/v).
2. Faka isixazululo sesinyathelo 1 ku-RT ku-600 rpm imizuzu engama-30 ukuze uqinisekise ukuhlanganisa okuphelele.
3. Hlanganisa isisombululo sesinyathelo sesi-2 ne-Adapter strand 2 – Rc – lfs (200 nM) ngesilinganiso esingu-1:1 (v/v).
   Isixazululo sesilungele ukusetshenziswa ekusebenzeni kwe-biochip.
   Ukuzinza kwesixazululo kuhlobene nokuzinza kwe-molecule ye-ligand.

Ithebula 2. Izinto ezengeziwe zokusebenzisa indawo 1 nendawo eyinkomba 2.

Okubalulekile	Ukugxila	Ibhafa	Igama Lomkhiqizo Ohlobene	I-oda No
I-adaptha umucu 1 – Rc	400 nM	I-TE40 [1]	I-adapter strand 1 ngodayi obomvu Rc	AS-1-Rc
I-Ligand umucu ethwele umsipha ohlangene	500 nM	2P] E40 [	I-heliX® Amine Coupling Kit 1	I-HK-NHS-1

Example
Ivolumu edingekayo yokusebenza oku-3: 100 μL nokugxiliswa kokugcina kwe-100 nM.

I-Vial 1		I-Vial 2
I-adaptha umucu 1 – Rc (400 nM)	Kuhlanganiswe Intambo ye-Ligand (500 nM)	I-adaptha umucu 1 – Rc – lfs (200/250 nM)
25µL	25µL	50µL

Ngemuva kwesikhathi sokufukamela, hlanganisa ibhodlela 1 kanye nebhodlela 2 ukuze uthole i-100 μL yesisombululo se-DNA esilungele ukusetshenziswa.

Oxhumana naye

I-Dynamic Biosensors GmbH
I-Perchtinger Str. 8/10
81379 eMunich
EJalimane

Inkampani engu-Dynamic Biosensors, Inc.
300 Trade Center, Suite 1400
I-Woburn, i-MA 01801
USA

Oda Ulwazi order@dynamic-biosensors.com
Ukusekela kwezobuchwepheshe support@dynamic-biosensors.com
www.dynamic-biosensors.com

Izinsimbi nama-chips akhiwe futhi akhiqizwa eJalimane.
©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. Wonke amalungelo agodliwe.

1. TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA
2. Uma iphrotheni ingazinzile ku-PE40 (TE40, HE40), sicela uhlole ukuhambisana kwebhafa neshidi lokuhambelana le-switchSENSE®.

www.dynamic-biosensors.com

Amadokhumenti / Izinsiza

	I-BIOSENSORS heliX enamandla kanye ne-Adapter Strand 2 [pdf] Imaniwali yosebenzisayo AS-2-Rc-lfs v5.1, heliX plus Adapter Strand 2, Adapter Strand 2, Strand 2
	I-BIOSENSORS heliX enamandla kanye ne-Adapter Strand 2 [pdf] Imaniwali yosebenzisayo i-heliX kanye ne-Adapter Strand 2, i-heliX plus, i-Adapter Strand 2, i-Strand 2

Izithenjwa

• Imaniwali yosebenzisayo