I-BIOSENSORS enamandla AS-2-Rc Imanuwali Yomsebenzisi Yesistimu Yokuhlaziya Ilabhorethri Ezenzakalelayo

Isitoreji: Ikepisi elimhlophe, sicela ubone idethi yokuphelelwa yisikhathi kulebula. Gwema imijikelezo eminingi yokuqandisa ngokucaphuna i-nanolever.

Imiyalo yokusetshenziswa komkhiqizo

Ukulungiselela | MIX&RUN

Hlanganisa umucu we-Adapter 1 – Rc (400 nM) kanye ne-conjugated Ligand strand ne-ligand 1 (500 nM) ku-1:1 isilinganiso (v/v).
Hlanganisa umucu we-Adapter 2 – Rc (400 nM) kanye ne-conjugated Ligand strand ne-ligand 2 (500 nM) ku-1:1 isilinganiso (v/v).

Fukamisa ngokuhlukene izixazululo ezimbili zesinyathelo 1 no-2 ekamelweni lokushisa ku-600 rpm imizuzu engu-30 ukuqinisekisa ukuhlanganisa okuphelele.
Hlanganisa izixazululo zesinyathelo 1 no-2 ngesilinganiso esingu-1:1 (v/v). Isixazululo silungele ukusebenza kwe-biochip.

Izici Eziyinhloko

I-Adapter strand 2 yokusebenza kwe-HeliX® Adapter Chip Spot 2.
Ihambisana ne-heliX® Adapter Chip.

Kufaka phakathi imicu ye-Adapter yokwenziwa kabusha okungu-50.
Ilungele i-MIX&RUN sample ukulungiselela.

I-Adapter strand 2 iphethe udayi obomvu we-hydrophobic (Rc) onokushaja okuphakathi nendawo.

I-heliX® Adapter Chip Overview

Izindawo ezi-2 ezinokulandelana kwesikhonkwane oku-2 okuhlukile kwekheli elinekhodi ye-DNA.

Incazelo Yomkhiqizo

Oda Inombolo: AS-2-Rc

Ithebula 1. Okuqukethwe kanye Nokwaziswa Kwesitoreji

Okokusetshenziswa kocwaningo kuphela.
Lo mkhiqizo unomkhawulo wempilo yeshelufu, sicela ubone idethi yokuphelelwa yisikhathi kulebula.

Ukuze ugweme imijikelezo eminingi yokuncibilika kweqhwa sicela uliquot i-nanolever

Ukulungiselela | MIX&RUN

I-in-solution hybridization ye-adaptha nemicu ye-ligand:

Hlanganisa umucu we-Adapter 1 – Rc (400 nM) kanye ne-conjugated Ligand strand ne-ligand 1 (500 nM) ku-1:1 isilinganiso (v/v).

Hlanganisa umucu we-Adapter 2 – Rc (400 nM) kanye ne-conjugated Ligand strand ne-ligand 2 (500 nM) ku-1:1 isilinganiso (v/v).
Fukamisa ngokuhlukene izixazululo ezimbili zesinyathelo 1 nesi-2 ku-RT ku-600 rpm imizuzu engama-30 ukuze uqinisekise ukuhlanganisa okuphelele.
Hlanganisa isisombululo sesinyathelo 1 no-2 ngesilinganiso esingu-1:1 (v/v). Isixazululo sesilungele ukusetshenziswa ekusebenzeni kwe-biochip. Ukuzinza kwesisombululo kuhlobene nokuzinza kwama-molecule e-ligand.

Ithebula 2. Izinto ezengeziwe zokusebenzisa indawo 1 nendawo eyinkomba 2.

Example
Ivolumu edingekayo yokusebenza oku-3: 100 μL nokugxiliswa kokugcina kwe-100 nM.

Ngemuva kwesikhathi sokufukamela, hlanganisa ibhodlela 1 kanye nebhodlela 2 ukuze uthole i-100 μL yesisombululo se-DNA esilungele ukusetshenziswa.

Oxhumana naye

I-Dynamic Biosensors GmbH Perchtinger Str. 8/10 81379 Munich Germany

Inkampani engu-Dynamic Biosensors, Inc.
300 Trade Center, Suite 1400 Woburn, MA 01801 USA

Oda Ulwazi order@dynamic-biosensors.com
Ukusekela kwezobuchwepheshe support@dynamic-biosensors.com
www.dynamic-biosensors.com

Izinsimbi nama-chips akhiwe futhi akhiqizwa eJalimane.

I-TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA

Uma iphrotheni ingazinzile ku-PE40 (TE40, HE40), sicela uhlole ukuhambisana kwebhafa neshidi lokuhambelana le-switchSENSE®.

www.dynamic-biosensors.com

FAQ

Q: Iyini impilo yeshalofu yomkhiqizo?
IMP: Umkhiqizo unomkhawulo wempilo yeshelufu, sicela ubheke idethi yokuphelelwa yisikhathi kulebula.

Umbuzo: Kufanele ngiwugcine kanjani umkhiqizo ukuze ngigcine uzinzo?
A: Gcina umkhiqizo ngekepisi elimhlophe ukuze ugweme ukuchayeka ekukhanyeni. Gwema imijikelezo yokuncibilika ngokuqandisa i-nanolever.

Q: Kufanele ngilungiselele kanjani isixazululo se-biochip functionalization?
A: Landela izinyathelo zokulungiselela i-MIX&RUN enikezwe kumanuwali yomsebenzisi ukuze ulungiselele isisombululo sokusebenza kwe-biochip.

Amadokhumenti / Izinsiza

I-BIOSENSORS enamandla AS-2-Rc Isistimu Yokuhlaziya Ilabhorethri Ezenzakalelayo Ngokugcwele [pdf] Imaniwali yosebenzisayo
I-AS-2-Rc, AS-2-Rc Uhlelo Lokuhlaziya Ilabhorethri Elizihambela Ngokugcwele, Uhlelo Lokuhlaziya Ilabhorethri Ngokugcwele, Uhlelo Lokuhlaziya Ilabhorethri, Uhlelo Lokuhlaziya Ilabhorethri, Uhlelo Lokuhlaziya, Isistimu

Izithenjwa

Imaniwali yosebenzisayo

I-BIOSENSORS enamandla AS-2-Rc Isistimu Yokuhlaziya Ilabhorethri Ezenzakalelayo Ngokugcwele