Shuttle Box Salinity User Guide

Okuqukethwe fihla

1 I-Shuttle Box Salinity

2 UKUSEBENZISA OKOKUQALA

3 UKULINGANISWA, UKUHLANZA, KANYE NENKONDLO

4 MISA UMHLAHLANDLELA

I-Shuttle Box Salinity

UKUSEBENZISA OKOKUQALA

Ukusethwa kwethangi. Beka ithange le-shuttle kanye nama-buffer amathangi ezingeni eliphelele kanye netafula eliqinile elingakwazi ukusekela isisindo samathangi ngamanzi kuwo. Beka amathangi ebhafa eduze kwethangi le-shuttle ukuze ugcine ubude beshubhu buncane. Vumela isikhala samaphampu, amashubhu (gwema ama-kinks), nezixhumi.
Ukusethwa kabusha kwe-loop. Sebenzisa i-schematic (Umhlahlandlela Wokusetha I, Ukujikeleza kabusha) ukuze usethe iluphu yokujikeleza kabusha. Amaphampu kufanele axhunywe ezindaweni eziphansi futhi aqhubeke ngokuqhubekayo (2, R1 + R2).
Khulisaasing kanye nesinqumoasing loop setup. Use the schematic (Setup Guide I, Increasing + Decreasing) to set up the increasing kanye nesinqumoasing loop. The pumps for the increasing (2, P1 + P3) and decreasing loop (2, P2 + P4) connect to the PowerX4. Place P1 + P3 in the high saline bath and P2 + P4 in low saline bath (Setup Guide I). The two baths (not included) should have sufficient capacity to support your experiments. The buffer tank comes with six fittings for tube connections. Four zisetshenziselwa i-Shuttle box system ye-oxygen. Vala okusele ngocezu olufushane lweshubhu (Umhlahlandlela Wokusetha I).
Ukubekwa kwenzwa. Beka ama-conductivity probe (TetraCon 325) ngqo ngaphakathi kwamathange ebhafa, futhi uqiniseke ukuthi ichopho le-probe limbozwe amanzi ageleza emathangeni (Setup Guide II, Izinzwa).
Ukubekwa kwekhamera. Ikhamera kufanele ibekwe phakathi nendawo futhi ibekwe phezulu ngokwanele ngaphezu kwethangi le-shuttle ukuze ithole indawo view okuhlanganisa yonke indawo lapho isilwane singahambahamba khona (Umhlahlandlela Wokusetha II, Ikhamera). Uma usebenzisa amathangi amaningi, sebenzisa ikhamera eyodwa ithangi ngalinye.
KUBALULEKILE. Uma ikhamera isibekwe ngendlela efanele, akumele inyakaze njengoba kuzodingeka wenze ukulinganisa kwephikseli okusha.
Izimo zokukhanyisa. Sincoma ukusebenzisa ukukhanya okungemuva kwethangi le-shuttle (okufana nephaneli yokukhanya ye-IR ebekwe ngaphansi kwethangi) ukuze kuthuthukiswe umehluko phakathi kwesilwane nengemuva (6). Ukukhanyisa ngaphezulu kungabangela ukubonakala kwamanzi, futhi kungase kuthinte nokuziphatha kwezilwane. Qiniseka ukuthi unezikhala phakathi kwephaneli yokukhanya ye-IR kanye nethangi le-shuttle ukuze ugweme imiphumela yokushisa engadingeki njengoba amaphaneli okukhanya e-IR angashisa ngesikhathi sokusebenza (Umhlahlandlela Wokusetha II, Ikhamera).
Ilungisa izinga lokugeleza. Hlola ukuthi isiqondiso sokugeleza silungile (Umhlahlandlela Wokusetha I, Ukujikeleza kabusha). Ukuze uthole ukuhlukaniswa okuhle kokugeleza, kubalulekile ukuthi womabili amagumbi amancane ethangi le-shuttle anikezwe amazinga okugeleza alingana ncamashi. Sincoma isivinini samanzi esingu-5 cm/sec ku-shuttle tank.
Ukuze ulungise izinga lokugeleza (Umhlahlandlela Wokusetha II, Lungisa ukugeleza):
1. Phakamisa noma wehlise indawo ye-buffer tank uma kuqhathaniswa ne-shuttle tank.
2. Sebenzisa i-tube clamps kumashubhu asuka empompini aphinde ajikelezwe kabusha aye emathangini esiviva.
Gwema ukuxuba. Ukuze ugweme ukuxubana phakathi kwama-sub-compartments amabili, kubalulekile ukuthi izinga eliphezulu lamanzi lilingane phakathi kwezinhlangothi ezimbili, okungukuthi, ukugwema amanzi asuka kolunye uhlangothi engena kwelinye ngenxa yokuhluka kwengcindezi (amandla adonsela phansi). Ukwehlukaniswa kokugeleza phakathi kwamagumbi amabili angaphansi kungabonwa ngeso lengqondo ngokwengeza udayi onemibala (isb, iwayini elibomvu noma umbala wokudla) endaweni eyodwa encane bese uwulandela ngokuhamba kwesikhathi (8).
Isinyathelo esilandelayo. Manje bheka umhlahlandlela osheshayo - ShuttleSoft v3 mayelana nendlela yokusetha izinsimbi ku-ShuttleSoft v3 kanye nendlela yokusebenzisa isofthiwe ngesistimu yebhokisi le-shuttle.

UKULINGANISWA, UKUHLANZA, KANYE NENKONDLO

10 pH -> CO₂ ukuguqulwa: Linganisa (12) i-WTW SenTix HWD pH probe ngaphambi kokuguqulwa. Lungiselela amanzi amathathu sampokuncane kwekhwalithi efanayo (okungukuthi umthamo webhafa) njengamanzi asetshenziselwa ukuhlola, futhi ugcine izinga lokushisa lamanzi lingashintshi futhi lifana. Sebenzisa iphampu yokuxuba igesi (Wösthof), amaseli okugeleza okukhulu, noma ingxube yegesi eseyenziwe ngomumo ukuze ubhubhule amasekhondi amathathu.ampkancane kumaleveli amathathu e-pCO₂ ahlukene phakathi kwebanga lezinga lokuhlolwa kwakho kwe-min/max pCO₂. Faka i-SenTix HWD pH probe endaweni yokuqala yamanziample, chofoza i ukukala i-pH, bese uthayipha ezingeni le-pCO₂ elihambisanayo. Chofoza + ukuze wengeze iphoyinti lokulinganisa elilandelayo (10). Yenza izilinganiso ezintathu ngamanzi sample ukuqinisekisa ukufundwa okuzinzile. Ukuguqulwa manje sekwenziwe.

11 Ulinganisa nini… Ngokujwayelekile, kufanele wenze ukulinganisa kwenzwa entsha uma uhlangabezana nezinkinga zokusebenza kwenzwa (isb. i-signal drift). Uma kungenjalo, ukulinganisa okusha kuyanconywa:

I-WTW Oxi 3310 (CellOx 325): Uma uphawu lophenyo lubaneka (ngemuva kokuthi isikhawu sokulinganisa esisethiwe sesiphelelwe yisikhathi (izinsuku eziyi-150 njengezijwayelekile)) noma uma usebenzisa i-probe entsha.
I-WTW Cond 3310 (TetraCon 325): Njalo ezinyangeni eziyisi-6 noma uma usebenzisa uphenyo olusha.
I-WTW pH 3310 (SenTix HWD): Uma uphawu lophenyo lubaneka (ngemuva kokuthi isikhathi sokulinganisa esisethiwe sesiphelelwe yisikhathi (izinsuku ezingu-7 njengezinga elijwayelekile) noma uma usebenzisa uphenyo olusha.
Witrox: Lapho % umoya uhlezi. ileveli ihamba nge-> +/-2 % noma lapho usebenzisa inzwa entsha yomoya-mpilo. Izinga lokushisa. i-probe ilinganiswe ngaphambilini futhi ayikwazi ukulinganiswa mathupha.

12 Linganisa uphenyo lwe-WTW: Sicela ubheke imanuwali efanele yomsebenzisi imitha ye-WTW ukuze uthole iziqondiso ezengeziwe. Imanuwali ngayinye ingatholakala kokuthi: loligosystems.com/manuals

13 Linganisa inzwa ye-Witrox oxygen: Ku-ShuttleSoft v3 > Imenyu enkulu > Ukulinganisa > Khetha ithuluzi le-Witrox. Chofoza esiteshini esifanele ( CH1-CH4) ukuze uvule imenyu yokulinganisa isiteshi (13). Khetha uhlobo lokufakwa kwezinga lokushisa (i-Witrox ilawulwa noma ichazwe ngumsebenzisi) bese wenza ukulinganisa kwamaphoyinti angu-2 Manuwali (13 kanye no-13.1):

a. Beka ithiphu lenzwa emanzini axubile anokulingana komoya sample. Lokhu kungafezwa ngokuhlanza umoya womkhathi ku-sampamanzi, isb ngepompo yomoya.
b. Lindela ukufundwa kwesigaba (isignali yenzwa) ukuze kuzinze bese uchofoza okuthi Funda amanani amanje ukuze ulondoloze inani lamanje njengenani lokulinganisa ELIPHEZULU (100 % ukugcwaliswa komoya).
c. Dlulisela inzwa emanzini angenawo umoya-mpilo sample, isb ngokuhlanza igesi ye-nitrogen ibe yi-sample amanzi noma ngokuncibilikisa ~ 10 amagremu we-Na2SO3 ku-500 ml wamanzi acwecwe.
d. Linda ukuthi ukufundwa kwesigaba kuzinze bese uchofoza okuthi Funda amanani amanje ukuze ulondoloze amasiginali enzwa wamanje njengenani lokulinganisa ELIPHANSI (0 % ukugcwala komoya).

14 Indlela yokuhlanza… Ngokuvamile, hlanza insimbi ngayinye kanye nenzwa phakathi kokusetshenziswa.

I-WTW probes: Sicela ulandele imiyalelo yokuhlanza enikezwe kumanuwali ye-CellOx 325, TetraCon 325, noma i-SenTix HWD probe. Imanuwali ngayinye ingatholakala kokuthi: loligosystems.com/manuals
Inzwa ye-Witrox oxygen: Sebenzisa insipho epholile noma i-bleach, futhi ugeze ngamanzi e-demi. Bese womisa (14).
Amamitha e-WTW kanye ne-Witrox: Sula ingaphandle lensimbi yokulinganisa ngesikhangisoamp, indwangu engenalutho. Khipha amagciwane endlini nge-isopropanol njengoba kudingeka. Gwema ukuthintana ne-acetone noma izihlanzi ezifanayo eziqukethe izincibilikisi.

15 Indlela yokugcina... Gcina zonke izinzwa endaweni emnyama, eyomile phakathi kokuhlolwa. Gwema ukuveza udayi we-fluorescent kunzwa ye-oxygen ye-Witrox ekukhanyeni kwe-UV. Ukukhanya kwe-UV kuzofiphaza udayi wezinzwa futhi kunciphise amandla esiginali (ampamandla).