FLUIGENT FLOW UNIT Izinzwa Zokugeleza Okubili

IZINYATHELO ZOKUZIVIKELA

Ungavuli i-Flowboard kanye namadivayisi we-FLOW UNIT. Sicela udlulisele konke ukusevisa emnyangweni wesevisi yangemuva kokuthengisa (support@fluigent.com) Vimbela noma yiziphi izinto noma uketshezi ekungeneni ku-Flowboard naku-FLOWUNITs, lokhu kungase kubangele ukwehluleka kwesiyingi esifushane noma okunye ukungasebenzi.Ukwehluleka ukuhlonipha lesi seluleko kungenza:

• Ziveze ku-direct current/voltage uma ngabe idivayisi ingaphansi kwevolumutage okungaholela ekulimaleni okukhulu
• Iwaranti yedivayisi eyize
• Khipha inkampani yethu kunoma yisiphi isikweletu esiphathelene nokulimala ngokomzimba noma kwedivayisi.

Ungabeki umkhiqizo endaweni engazinzile enezinga eliphezulu kanye nokwesekwa okuqinile nokuzinzile Ungasebenzisi okunye ukunikezwa kwamandla kagesi kunaleyo enikeziwe, kukhethwe ngokucophelela ukuze kuhlangatshezwane nezidingo zamandla ze-Flowboard kukho konke ukucushwa kanye nokuthobelana nayo. zonke izindinganiso zokuphepha. Ububanzi be-FLOW UNIT XS capillary buncane: 25 µm. Hlunga isixazululo sakho, uma kungenzeka engeza isihlungi kumzila we-fluid futhi uhlanze i-FLOW UNIT XS ngemva kokusetshenziswa ngakunye.

ISINGENISO

Ububanzi be-FLOW UNIT buhlinzeka ngesixazululo sokulinganisa kanye/noma sokulawula ukugeleza kwanoma yiziphi izinhlelo zokusebenza eziwuketshezi. Ukuhlanganisa i-FLOW UNIT nohlelo lwethu lokusingatha ingcindezi (i-Flow EZTM noma i-Flowboard ehlanganiswe ne-MFCSTM) kuzokunikeza ithuba lokuhlola ngaso sonke isikhathi izinga lokugeleza kanye nevolumu yoketshezi olugeleza ohlelweni lwakho olugcwele amanzi. Amamodeli amane ahlukene e-FLOW UNIT anikeza ukukhetha okunabile kwebanga lokugeleza ukuze kufane kangcono nokunemba okudingekile, ukusuka ku-8 nL/min ukuya ku-40 mL/min. Eceleni kwezixazululo ezisekelwe emanzini, ukulinganiswa kwesibili kwama-hydrocarbon kuyatholakala kumamodeli amathathu (3) e-FLOW UNIT ahlukene (S, M+ kanye ne-L+), bheka §8. . Izochaza konke ukusebenza kweyunithi ye-Flow futhi izokusiza ukuthi uxhume wonke amamodeli e-FLOW UNIT ahlukene futhi uyisebenzise nazo zonke izinto zokusebenza: nge-Fluigent Flow EZTM kanye ne-MFCSTM-EZ

ulwazi oluvamile

Isimiso sobuchwepheshe
I-Flow Unit inika amandla ukukalwa kwezinga lokugeleza, ebangeni elibanzi lamazinga okugeleza ngenxa yamamodeli amahlanu (5): XS, S, M+, L+. Ukutholwa kwezinga lokugeleza kusekelwe kubuchwepheshe be-thermal. Isici sokushisisa ku-microchip sengeza isilinganiso esincane sokushisa esiphakathi nendawo ukuze kukale ukugeleza okushisayo. Izinzwa ezimbili zamazinga okushisa, ezitholakala ngokulinganayo ngenhla nangaphansi komthombo wokushisa, zibona ngisho nokuhluka okuncane kwezinga lokushisa, ngaleyo ndlela zinikeze ulwazi oluyisisekelo mayelana nokusabalala kokushisa, ngokwako okuhlobene ngokuqondile nokugeleza.

Le manuwali yomsebenzisi izokukhombisa ukuthi uwafaka kanjani futhi uwasebenzise kanjani amayunithi agelezayo emsebenzini wakho wansuku zonke. Izochaza zonke izici zokusebenza kweyunithi ye-Flow futhi izokusiza ukuthi uxhume wonke amamodeli we-FLOW UNIT engafanele futhi uyisebenzise nazo zonke izinto zokusebenza: ane-Fluigent Flow EZTM kanye ne-MFCSTM-EZFour (4) amamodeli e-FLOW UNIT ahlukene ayatholakala. Zincike kububanzi besilinganiso sokugeleza nasekulinganisweni. Nasi isithombe samamodeli ama-FLOW UNIT Amane (4) anobubanzi obuhlukene, nokulinganiswa okukabili ngakunye . Yonke imininingwane ye-fluidic ivezwe kuthebula lemininingwane.

Qaphela: I-FLOW UNIT ingasebenza ngendlela ehamba phambili ngayo ngezisombululo zokulawula ukuhamba kwengcindezi ye-FLUIGENT (FLOW EZ™ kanye ne-MFCS™-EZ). Imininingwane eyengeziwe ku www.fluigent.com.

Imininingwane

Sicela uqaphele ukuthi ukucindezela okukhulu kuncike kumodeli ye-FLOW UNIT. Qinisekisa ukuthi ingcindezi esetshenziswa ku-FLOW UNIT ayidluli leli nani ngaso sonke isikhathi.
I-FLOW UNIT ifanele isilawuli sakho samanzi. Uma usebenzisa isilawuli sokucindezela kungase kudingeke ukuthi ufake ukucindezela okukhulu ngaphansi kwaleli nani. Uma usebenzisa esinye isilawuli sokugeleza, qaphela ukuthi ukucindezela kungase kukhuphuke kunebha ye-100 kalula kakhulu futhi kungase kubangele umonakalo ku-FLOW UNIT yakho.

INCAZELO YEyunithi yokugeleza

FLOW UNIT ngaphambili nangemuva

1. Imodeli yenzwa
2. Ukulinganisa
3. Isiqondiso sesilinganiso sokugeleza esihle
4. Ibanga

Izimbobo ezimbili (2) ze-fluidic zisezinhlangothini zedivayisi. Ingaphambili le-FLOW UNIT libonisa ulwazi mayelana nobubanzi kanye nokulinganiswa: Uhlamvu lubonisa “imodeli”; Nansi i-S. Iconsi libonisa ukulinganiswa. Uma kukhona ithonsi elilodwa elimhlophe, Kubonisa ukuthi inzwa ilinganiselwe amanzi. Kodwa-ke uma kukhona ithonsi eliluhlaza okwesibhakabhaka elengeziwe likhombisa ukuthi kukhona ukulinganiswa okukabili kwamanzi kanye ne-Isopropyl alcohol Ingemuva le-FLOW UNIT libuye libonise ulwazi mayelana nobubanzi kanye nokulinganiswa: Uhlamvu lubonisa "imodeli"; Nansi i-S. Iconsi libonisa ukulinganiswa. Lapha kukhona ithonsi elilodwa elimhlophe: libonisa ukuthi inzwa ilinganiselwe amanzi kanye ne-IPA. Ububanzi buboniswa ngokucacile: 0 ± 7µL/min (amanzi) ; 0 ± 70µL/min (IPA)

Ukuxhumana okujwayelekile kwe-fluidic

XS / S amashubhu nokufakwa
Amamodeli we-XS kanye ne-S FLOW UNIT anezimbobo ezimbili (2) ezidonsa amanzi. Izici zalezo zimbobo ezimbili (2) yilezi: Usayizi wochungechunge: UNF 6-40. Ihambisana namashubhu angu-1/32'' ububanzi bangaphandle (1/32'' OD). Ukuze uqalise, u-FLUIGENT angakunikeza ikhithi ye-“CTQ_KIT_LQ” ehlanganisa:

• Umkhono owodwa (1) oluhlaza 1/16'' OD x 0.033''x1.6”
• Izixhumi ezimbili (2) zeyunithi yokugeleza ye-LQ ye-1/32''OD yamashubhu,
• Imitha eyodwa (1) ye-PEEK Tubing Blue 1/32'' OD x0.010'' ID
• I-adaptha eyodwa (1) i-PEEK 1/16'' ukuya ku-1/32'' ishubhu ye-OD

Qaphela: Njengoba kunezinhlobonhlobo zamashubhu kanye nokufakwa kwezinhlelo zokusebenza ezahlukene ongazisebenzisa, i-FLUIGENT ikweluleka ukuthi wenze isiqiniseko sokuthi isistimu yakho yokuxhumana okumanzi ilingana namachweba amabili (2) agelezayo e-FLOW UNIT. Uma kungenjalo, sicela uqaphele ukuthi kunephaneli enkulu yama-adaptha nezinyunyana zokuxhuma amashubhu akho kwewethu. Vakashela ku-www. uigent.com ukuze ufunde kabanzi mayelana nezinto zokwakha kanye ne-ID etholakalayo ne-1/32'' noma 1/16” OD amashubhu, amantongomane nama-ferrules avela kubahlinzeki bezinto zokufakelwa ukuze ivumelane nesicelo sakho.

XS/S uxhumano

1. Sika ishubhu elingu-1/32'' le-OD libe ngobude obufunayo, ushiye ubuso obusikwe isikwele.
2. Shelelisela ukufakwa phezu kweshubhu.
3. Faka ukuhlanganisa embobeni eyamukelayo, futhi ngenkathi ubambe ishubhu ngokuqinile ngaphansi kwembobo, qinisa umunwe wokulinganisa uqinise.
4. Ukuze uhlole ukucinana koxhumo lwakho, ungadonsa ngobumnene ishubhu: kufanele ihlale ifakwe ku-ferrule namantongomane.
5. Yenza okufanayo ku-2nd port.

Ishubhu ye-M+/L+ nokufakwa
Izinhlobo ze-M+ ne-L+ FLOW UNIT zinamachweba amabili agelezayo. Izici zalezo zimbobo ezimbili (2) yilezi: Usayizi wochungechunge: ¼-28. Uhlobo oluphansi phansi (FB). Ihambisana namashubhu angu-1/16'' ububanzi bangaphandle (1/16'' OD). Ukuze uqalise, u-FLUIGENT angakunikeza ikhithi ye-“CTQ_KIT_HQ” ehlanganisa:

• Izixhumi ezimbili (2) ze-Flow Unit HQ ¼-28 Flat
• Phansi kwe-1/16'' OD amashubhu
• Izithako ezine (4) zeyunithi yokugeleza ye-HQ
• 1 m FEP amashubhu 1/16'' OD * 0.020''ID

Qaphela: Njengoba kunezinhlobonhlobo zamashubhu kanye nokufakwa kwezinhlelo zokusebenza ezahlukene ongazisebenzisa, i-FLUIGENT ikweluleka ukuthi wenze isiqiniseko sokuthi isistimu yakho yokuxhumana okumanzi ilingana namachweba amabili (2) agelezayo e-FLOW UNIT. Uma kungenjalo, sicela uqaphele ukuthi kunephaneli enkulu yama-adaptha nezinyunyana zokuxhuma amashubhu akho kwewethu. Vakashela ku-www. uigent.com ukuze ufunde kabanzi mayelana nezinto zokwakha kanye ne-ID etholakalayo ne-1/32'' noma 1/16” OD amashubhu, amantongomane nama-ferrules avela kubahlinzeki bezinto zokufakelwa ukuze ivumelane nesicelo sakho.

Uxhumano lwe-M+/L+

1. Sika ishubhu elingu-1/16'' le-OD libe ngobude obufunayo, ushiye ubuso obusikwe isikwele.
2. Shelelezisa inadi phezu kweshubhu intambo yamantongomane ibheke ekugcineni kweshubhu ixhunywe.
   Slip i-ferrule phezu kweshubhu, ingxenye ecijile ye-ferrule ibheke nati. QAPHELA: amantongomane nama-ferrules aklanyelwe ngqo ukusebenza ndawonye. (I-FLUIGENT ikweluleka ukuthi uhlobanise kuphela ama-ferrules anikeziwe namantongomane anikeziwe futhi ngokuphambene nalokho).
3. Faka ukuhlanganisa embobeni eyamukelayo, futhi ngenkathi ubambe ishubhu ngokuqinile ngaphansi kwembobo, qinisa umunwe wentongomane.
4. Ukuze uhlole ukucinana koxhumo lwakho, ungadonsa ngobumnene ishubhu: kufanele ihlale ifakwe ku-ferrule namantongomane.
5. Yenza okufanayo ku-2nd port.

UKUSETHA NGE-FLOW EZTM

Incazelo ye-EZTM egelezayo
I-Flow EZ™ iwuhlelo oluthuthuke kakhulu olutholakalayo lokulawula ukugeleza okusekelwe kwingcindezi. Idivayisi ehlangene ima eduze kwedivayisi encane, evumela umsebenzisi ukuthi anciphise ukusetshenziswa kwesikhala sebhentshi ngaphandle kwesidingo se-PC. Enye ingasebenza futhi ikhiqize idatha ngokushesha. I-Flow EZ™ isekela osayizi be-reservoir ukusuka ku-2 mL ukuya kumabhodlela aselabhorethri ilitha ilitha. Umuntu angasebenzisa amachibi amakhulu futhi alondoloze ukugeleza okuqhubekayo, okungapheli izinsuku ngaphandle kokugcwalisa.

Ihlanganiswe ne-FLOW UNIT ivumela ukufinyelela ngesikhathi sangempela isilinganiso sokugeleza nokulawula kusistimu yakho.

Ukuxhuma ku-Flow EZTM
Ukuze uxhume i-FLOW UNIT ku-Flow EZTM mane uxhume ikhebula le-USB ukusuka ku-FLOW UNIT ukuya ku-Flow EZTM.

1. FLOW UNIT (inzwa)
2. I-FLOW EZTM (Isilawuli sokugeleza esisekelwe ekucindezelekeni)

Ukuze uthole ulwazi olwengeziwe mayelana nendlela yokuxhuma nokusebenzisa i-Flow EZTM hlola yethu webikhasi kanye nemanuwali yomsebenzisi we-Flow EZTM https://www.fluigent.com/research/instruments/pressure-flow-controllers/lineup-series/flow-ez/ Uma isixhunywe ku-Flow EZTM kanye nohlelo lwe-fluidic (indawo yokugcina amanzi kanye ne-chip) izinga lokugeleza lingalinganiswa ngokuqondile ku-Flow EZTM kumodi yendawo noma ngokusebenzisa i-OxyGEN.

Imodi yendawo: kala futhi ulawule izinga lokugeleza

Isilinganiso sokugeleza

Uma i-FLOW UNIT isixhunyiwe, idivayisi iyithola ngokuzenzakalelayo futhi "iwindi lokusebenza" lizobonisa indawo eyengeziwe okuhlanganisa nesilinganiso sokugeleza. Izinga lokugeleza elilinganisiwe (Qmeas) liyizinjongo zokuqapha kuphela. Ukuze ulawule ngokuqondile izinga lokugeleza, bheka ikhasi elilandelayo (Ukulawula izinga lokugeleza)

Kulokhu kulungiselelwa uzokwazi ukufinyelela esilinganisweni sezinga lokugeleza ngesikhathi sangempela. Ungabe usulungisa ingcindezi ukuze ufinyelele izinga lokugeleza ofuna ukuliqondisa.

1. Uhlobo loketshezi H2O noma i-Isopropanol
2. Ibanga le-FLOW UNIT kuye ngezinga lokugeleza okuqondiwe (XS, S, M+, L+)
3. Shintshela kumodi yokulawula izinga lokugeleza bona ikhasi elilandelayo
4. Amayunithi wesilinganiso sokugeleza esikaliwe angashintshwa kusetshenziswa imenyu
5. Umyalo wengcindezi ozosethwa umsebenzisi

Ukulawula izinga lokugeleza

Uma i-FLOW UNIT ixhunyiwe, cindezela inkinobho yesokunxele ethi “Setha u-Q Ctrl” ukuze ushintshele kumodi yokulawula izinga lokugeleza.

1. Amayunithi wesilinganiso sokugeleza esikaliwe abonisiwe angakhethwa
2. Umyalo wesilinganiso sokugeleza ozosethwa umsebenzisi
3. Buyela emuva kumodi yokulawula ingcindezi

Umsebenzisi angakwazi ukulawula izinga lokugeleza ngokuqondile, ngokusetha umyalo wezinga lokugeleza (Qcmd) Nakuba imodi yokulawula ikuzinga lokugeleza, inani lesigaba sokucindezela okubukhoma ku-reservoir (Pmeas) lisaboniswa phakathi, linikeza ulwazi mayelana nokugeleza kwamanzi. ukusetha. Amazinga okugeleza angavamile angase abonise izinkinga ekusethweni kwe-microfluidic (ukuvuza, ukuvala, njll.)

I-Oxygen: kala futhi ulawule izinga lokugeleza

Isilinganiso sokugeleza
Ukuze ulawule usebenzisa isofthiwe ye-OxyGEN imojuli yesixhumanisi kufanele yengezwe ekusetheni: Imojula yesixhumanisi iyimojuli evumela ukuxhumana phakathi kwe-Flow EZ nekhompyutha. Ukuze uthole ulwazi olwengeziwe sicela ubheke kumanuwali yomsebenzisi wohlelo :  https://www.fluigent.com/resources-support/support-tools/downloads/user-manuals/lineup-series-user-manual/ Imojula yesixhumanisi kufanele ixhunywe ku-Flow EZ kuqala. Uma Isixhumanisi sixhunywe ku-Flow EZ, hlanganisa iyunithi ye-Flow ku-Flow EZ.

Ngemuva kokuthi iyunithi yokugeleza ixhunywe ngempumelelo ku-Flow EZ ukuze ulinganise futhi ulawule izinga lokugeleza udinga nje ukwethula isofthiwe ye-Oxygen.

Isofthiwe ye-Oxygen izothola ngokuzenzakalelayo ithuluzi elixhunywe ebhodini lokugeleza futhi ibonise ngokushesha isilinganiso sezinga lokugeleza seyunithi ngayinye yokugeleza exhunyiwe kumagrafu wesilinganiso sokugeleza.

Igrafu yezinga lokugeleza

Igrafu yesilinganiso sokugeleza ibika izilinganiso zenzwa yamanje yezinga lokugeleza . Uma kudingeka ukulawulwa kwezinga lokugeleza kungenzeka uchofoze kusithonjana Sesandla ukuze uqalise i-DFC (Imodi yokulawula ukugeleza okuqondile). Ngemuva kokuthi iyunithi yokugeleza ixhunywe ngempumelelo ku-Flow EZ ukuze ulinganise futhi ulawule izinga lokugeleza udinga nje ukwethula isofthiwe ye-Oxygen.

I-oda elisha linganikezwa kusetshenziswa ikhesa eqondile uma i-DFC isethiwe amagrafu wesilinganiso sokugeleza noma njengenombolo kunkambu yombhalo enikelwe. Umuntu angashintsha iyunithi yesithenjwa ngebhokisi elikhethiwe ngaphansi kwenkambu ethi “Oda”. Igama lesiteshi (elingashintshwa) nezici zaso zingabonakala ekhoneni eliphezulu kwesokudla. Ukuze uthole imininingwane eyengeziwe sicela ubheke imanuwali yomsebenzisi we-Oxygen kulesi sixhumanisi esilandelayo: https://www.fluigent.com/resources-support/support-tools/downloads/user-manuals/

Ukutholwa kwebhamuza
Uma kutholwa umoya, ama-eras abomvu azovezwa ku-flowgraph phakathi nesikhathi sokutholwa.

UKUSETHA NGEFLOWBOARD

Ukuze sisebenzise uhla lwezinzwa zethu ze-FLOW UNIT ngaphandle kwe-Flow EZTM i-Flowboard iwumkhiqizo okufanele usetshenziswe. Le divayisi isingatha amamodeli afika kwangu-8 (XNUMX) FLOW UNIT futhi iwahlinzeka ngogesi. I-Flowboard futhi iyisixhumanisi phakathi kwamamodeli e-FLOW UNIT axhunyiwe nesofthiwe ye-OxyGEN. Lapho uhlanganisa i-FLOW UNIT ne-MFCSTM-EZ, umuntu kufanele asebenzise isofthiwe ye-OxyGEN.

Incazelo ye-Flowboard

I-Flowboard iyihabhu enika amandla futhi ixhumane phakathi kwe-Fluigent Software kanye nama-FLOW UNIT afika kwayisishiyagalombili.Asebenza njenge-Flow-Rate Platform ukuze alinganise futhi abonise amazinga okugeleza ngesikhathi sangempela. I-Flowboard iyadingeka ekulawuleni izinga lokugeleza uma usebenzisa isilawuli sokugeleza kochungechunge lwe-MFCS™. Ingasetshenziselwa ukulinganisa nokubonisa izinga lokugeleza nganoma iyiphi isistimu yokulawula ukugeleza.

1. Inkomba eluhlaza (i-LED yamandla) iyakhanyisa lapho i-FLOWBOARD ixhunyiwe.
2. Imbobo ye-USB (uhlobo B) ixhuma i-FLOWBOARD kukhompyutha ukuze ilawule isofthiwe
3. Kunezimbobo ezincane ze-USB eziyisishiyagalombili (8) (ukuxhuma kufika kumadivayisi ayisishiyagalombili (8) e-FLOW UNIT).

Ngemuva kwe-FLOWBOARD ithebula lifingqa wonke amamodeli we-FLOW UNIT atholakalayo nezici zawo. Phansi kwe-FLOWBOARD ilebula ekhombisa inombolo yomkhiqizo, inombolo ye-serial, yamanje kanye nevolumutage.

Ixhuma ku-Flowboard ne-PC

Uxhumano lwe-USB
Xhuma ipulaki yohlobo B yekhebula le-USB elihlinzekwe ne-Flow- Rate Platform ohlotsheni B lwembobo ye-USB ngaphambili kwe-FLOWBOARD.Xhuma enye ingxenye yentambo ye-USB (uhlobo A ipulaki evamile) kukhompyutha lapho isofthiwe ehambisanayo. ifakiwe

Uxhumano lwe-FLOW UNIT
Ukuze uxhume i-FLOW UNIT ku-FLOWBOARD, xhuma isiphetho sepulaki ye-USB encane ehlanganiswe ne-FLOW UNIT kwesinye sezimbobo ze-USB ezincane eziyisishiyagalombili (8) ku-FLOWBOARD.

Umhlahlandlela wokuqalisa osheshayo

1. Okokuqala, ungase ufune ukuhlanganisa i-FLOW UNIT ehlukile ohlelweni lwakho lwe-microfluidic, nokufakwa okufanele.
2. Bese, xhuma amamodeli we-FLOW UNIT ku-FLOWBOARD.
3. Bese uxhuma i-FLOWBOARD kanye nekhompyutha ngekhebula le-USB.
4. Ukuze uqedele, qala isofthiwe (i-Oxyge ) efakwe kukhompyutha yakho (umanuwali yomsebenzisi) usebenzisa isixhumanisi esilandelayo : https://www.fluigent.com/resources-support/support-tools/software/oxygen/
5. Manje ungasebenzisa i-Flow-Rate Platform ohlelweni lwakho lokusebenza.

Ungakhohlwa ukuhlanza nokugeza i-FLOW UNIT yakho ngemuva kokuyisebenzisa.

Ibhodi eligelezayo: kala futhi ulawule izinga lokugeleza
Ngemuva kokuthi iyunithi yokugeleza kanye nebhodi lokugeleza kuxhunywe ngempumelelo, ukuze ulinganise futhi ulawule izinga lokugeleza udinga nje ukwethula isofthiwe ye-Oxygen. Isofthiwe ye-oxygen izothola ngokuzenzakalelayo ithuluzi elixhunywe ebhodini lokugeleza futhi ibonise ngokushesha isilinganiso sezinga lokugeleza seyunithi ngayinye yokugeleza exhunyiwe kumagrafu wesilinganiso sokugeleza.

Ukuze uthole imininingwane eyengeziwe sicela ubheke imanuwali yomsebenzisi we-Oxygen kulesi sixhumanisi esilandelayo: https://www.fluigent.com/resources-support/support-tools/downloads/user-manuals/

Igrafu yezinga lokugeleza
Igrafu yesilinganiso sokugeleza ibika izilinganiso zenzwa yamanje yezinga lokugeleza. Uma kudingeka ukulawula izinga lokugeleza ungachofoza kusithonjana Sesandla ukuze uqalise i-DFC (Imodi yokulawula ukugeleza okuqondile).

I-oda elisha linganikezwa kusetshenziswa ikhesa eqondile uma i-DFC isethiwe amagrafu wesilinganiso sokugeleza noma njengenombolo kunkambu yombhalo enikelwe. Umuntu angashintsha iyunithi yesithenjwa ngebhokisi elikhethiwe ngaphansi kwenkambu ethi “Oda”. Igama lesiteshi (elingashintshwa) nezici zaso zingabonakala ekhoneni eliphezulu kwesokudla.

Ukutholwa kwebhamuza
Uma kutholwa umoya, ama-eras abomvu azovezwa ku-flowgraph phakathi nesikhathi sokutholwa.

UKUKHALISA OKUMBILI

Isimiso sokulinganiswa okukodwa nokukabili

Amamodeli ahlukene we-FLOW UNIT ayalinganiswa ukuze anikeze ukufunda okunembile lapho esetshenziswa noketshezi oluhambisanayo, amanzi noma i-isopropyl alcohol. Kumodeli ye-FLOW UNIT XS, kuphela ukulinganisa okukodwa kwamanzi okutholakalayo. Kumamodeli we-FLOW UNIT S/M+/L+, ukulinganiswa okubili kuyatholakala: Amanzi kanye ne-Isopropyl alcohol. I-FLOW UNIT ingasetshenziselwa ukuphatha uketshezi oluhlukene olungalinganiselwe ekuqaleni. Uma kungenzeka, khetha inkundla yokulinganisa ejwayelekile efana kakhulu noketshezi lwakho olumanzi. Okwesiboneloample, ukulinganiswa kwamanzi kungasetshenziselwa isixazululo esisekelwe emanzini kanye nokulinganiswa kotshwala kwe-isopropyl kuma-hydrocarbon noma uwoyela. Ukulinganisa kungakhethwa futhi kushintshwe ku-software Ukuze kutholwe amanani okugeleza anembile kwezinye izinto eziwuketshezi, kuyadingeka ukusebenzisa izici zokulungisa (isici sesikali), ukuguqula inani elibonisiwe libe yinani langempela. Isici sesikali singengezwa kusofthiwe (bheka isici sesikali ngokwezifiso encwadini ehambisanayo yomsebenzisi). Ukwengeza isici sesikali kuqinisekisa ukuthi ukufundwa kwenzwa yokugeleza manje sekunembile kuketshezi oluqondiwe. Isigaba esilandelayo sichaza ukuthi ungayibala kanjani le factor futhi sibonisa i-ex.ample ngamafutha aqhakazile: FC-40.

Indlela yokulinganisa: ExampI-le ene-FC40 yokulinganisa uwoyela
Indlela yokuhlinzeka ngezinga lokugeleza elaziwayo liyadingeka ukuze kutholakale isici sesikali samanzi akhethiwe. Lokhu kungaba iphampu yesirinji, iphampu ye-peristaltic noma isilawuli sokucindezela esiletha uketshezi kubhalansi enembayo enevolumu ebalwa kusukela ekuminyaneni okwaziwayo. Nansi i-exampusebenzisa i-Flow EZTM, isilawuli sokugeleza esisheshayo nesizinzile esisekelwe kwingcindezi elethwa ngu-FLUIGENT. Inhloso yalobu buchwepheshe be-FASTABTM ukucindezela indawo yokugcina amanzi equkethe uketshezi lwenzuzo okumele ijovwe ngohlelo lwe-microfluidic. Yenza ithebula eliqukethe isikhathi sesilinganiso ngasinye, izinga lokugeleza lephampu kanye nedatha elinganiswa nge-FLOW UNIT. Kunconywa izilinganiso okungenani ezi-3 ngezinga lokugeleza ngakunye.

UKUKHALISA OKUMBILI
Umgomo wokuhlolwa uwukujova uketshezi olufunwayo, nansi i-FC-40, ngemodeli efiswayo ye-FLOW UNIT exhunywe ku-FlowEZ. Khona-ke ngesikhathi esifanayo urekhoda izinga lokugeleza elinikezwe isofthiwe futhi ukala isisindo soketshezi oluqoqile esikhathini esikhethiwe. Ngokwazi ukuminyana koketshezi, uyakwazi ukuchaza izinga lokugeleza langempela.

Qaphela ukuthi uma kusetshenziswa iphampu ye-peristaltic noma isirinji, umuntu kufanele alinde kuze kufinyelele izinga lokugeleza eliqondiwe (izikhathi zokumisa zingaba zinde) futhi abale isilinganiso sokugeleza kwesilinganiso ngenxa yokushaya.

Uhlu lwezinto ezidingekayo ukuze kukhiqizwe kabusha isilingo lunikezwe ngezansi:

• Okukodwa (1)FLOW EZ
• Imodeli eyodwa (1) FLOW UNIT
• Isikali esisodwa (1) sesisindo esinembayo

Ithebula elingezansi libonisa ulwazi olurekhodiwe ngesikhathi sokuhlolwa: ingcindezi ebekwe yi-MFCS™- EZ, Qs izinga lokugeleza elirekhodwe i-FLOW UNIT ngesofthiwe ye-Flow- Rate Platform, Qw izinga lokugeleza elikalwa ngesilinganiso sesisindo esinembayo. , kanye nama-Qw/Qs isici sesikali esibaliwe sokulinganiswa kwephoyinti elilodwa.

Ngakho-ke, lapho usebenza cishe ku-317 µl/min (izinga lokugeleza eliqondiwe), kufanele ungeze isici sesikali esingu-3.5 ukuze ukukalwa kwenzwa kuhambisane nezinga lokugeleza langempela le-FC-40.

INKQUBO YOKUHLANZA

Amamodeli we-FLOW UNIT azwela kakhulu futhi kufanele ahlanzwe kahle ukuze alondoloze ukusebenza okuphezulu njalo. Ngokunakekela nokunakekelwa okufanele, ama-Flow Units angahlala iminyaka eminingi. Akukho ukuhlanzwa noma ukuhlanzwa okungafanele okungashiya amadiphozithi odongeni lwangaphakathi lwe-capillary okungase kubangele ukuchezuka kokulinganisa ngisho nokuvala imbala. Ukuhlanza inzwa ngemva kokusetshenziswa nangaphambi kokugcina idivayisi isikhathi eside kufanele kuvimbele izinzwa kunoma yimuphi umonakalo.

Incazelo
Ngaphakathi kwezinzwa zokugeleza kwe-liquid, i-sensor chip ikala ukugeleza odongeni lwe-capillary yengilazi enezindonga ezacile. Ngenxa yokuthi ukulinganisa kusebenzisa ukusakazwa kokushisa ngodonga lwengilazi kanye nokushintshisana kokushisa nendawo, kubalulekile ukuthi ukuhlangana kwe-chip ne-medium kungashintshiwe. Ukwakhiwa kwamadiphozi odongeni lwengilazi ngaphakathi kwe-capillary kungase kuvimbele ukudluliswa kokushisa.

Ukuphatha okuvamile
Ungavumeli inzwa ukuthi yome ngemidiya eshubhu le-capillary ngaphandle kokucwenga kuhlanzeke kuqala. Zama futhi ukugwema ukuvumela inzwa egcwele ihlale isikhathi eside (kuya ngoketshezi lwakho). Ngaphambi kokugcina inzwa, hlala njalo ukhipha uketshezi, shayela nge-ejenti yokuhlanza, ukhiphe, futhi womise i-capillary. kumodeli ye-XS FLOW UNIT, hlunga isisombululo sakho ngesihlungi se-membrane esingu-5µm (noma esiphansi).

Inqubo
Ukuhlanzwa kanye nokushaywa kwamanzi kwama-Flow Units kufanele kucabangele uhlobo lwezinto ebeziphanjwa ngazo. Imvamisa, umuntu kufanele akhethe isixazululo sokuhlanza esiphephile ku-Flow Unit (indawo engaphakathi) futhi konke okunye okusethiwe okwamanje kuzoncibilikisa uhlobo lwe-s.ampkancane ezazithintana nobuso. Ku-Flow Unit XS, S kanye ne-M, uketshezi oluwuketshezi kufanele luhambisane nengilazi ye-PEEK ne-Quartz. Ku-Flow Unit M+ kanye ne-L+, uketshezi oluwuketshezi kufanele luhambelane ne-PPS, insimbi engagqwali (316L) Izinyathelo ezilandelayo ziyanconywa ezisombululweni ezisekelwe emanzini, ngendlela efanele: Hlanza lonke uhlelo lwakho ngamanzi Hlanza Iyunithi Egelezayo ngento engekho- okokugeza amagwebu. Isihlanzi sidinga ukusebenzisana ne-Flow Unit, yonke ingxenye yokusetha kwakho (i-microfluidic chip, ikakhulukazi) kanye noketshezi olusetshenziswa ngaphambili phakathi nokuhlola kwakho. Susa konke ukungcola ngenxa yesibulala-magciwane (isibample, i-Javel bleach). Hlanza i-Javel bleach (noma isibulala-magciwane esikhethiwe) ngamanzi. Hlanza lonke uhlelo lwakho nge-isopropanol. Ngenxa yalesi sinyathelo sokugcina, ngeke ushiye lutho ku-FLOW UNIT yakho. Bese, amapulaki aphuzi enzwa kufanele afakwe ukuze agcinwe.

Izincomo zoketshezi

Ukusebenza ngoketshezi oluningi
Ukushintsha phakathi koketshezi oluningi kungashiya amadiphozithi esikhashana esesimweni sezingqimba eziwuketshezi ngaphakathi kwe-capillary yengilazi. Lokhu kuvame kakhulu oketshezini olungancibiliki kodwa kungenzeka ngisho nezinhlanganisela zoketshezi oluxubile. Okwesiboneloample, lapho i-IPA ilandelwa amanzi kunzwa ngaphandle kokoma phakathi, ama-offset amakhulu angabonwa amahora ngemva kokushintshela emanzini. Uma kungenzeka, nikela inzwa ehlukile kuketshezi ngalunye oluhlukile oluzokalwa. Uma kungenzeki, qaphela lapho ushintsha imidiya futhi uhlanze kahle.

Ukusebenza ngamanzi
Uma usebenza ngamanzi kunconywa ukuthi ungavumeli inzwa yome. Wonke usawoti namaminerali emanzini azofaka engilazini futhi kube nzima ukuwakhipha. Nakuba izixazululo zikasawoti zivame kakhulu ezinkingeni, ngisho namanzi ahlanzekile asengaqukatha amaminerali ancibilikisiwe anele ukuze akhe ungqimba lwe-deposition. Geza ngamanzi e-DI njalo ukuze uvimbele ukwakheka. Uma usahlangabezana nezinkinga, ngezikhathi ezithile fafaza inzwa ngama-ejenti okuhlanza ane-acidic kancane.
Lapho usebenza ngamanzi aqukethe izinto eziphilayo (ushukela, njll.) ama-microorganisms avame ukukhula ezindongeni ze-glass capillary futhi enze ifilimu ephilayo okungaba nzima ukuyisusa. Geza njalo ngezincibilikisi ezifana ne-ethanol, i-methanol noma i-IPA, noma ngezinto zokuhlanza ukuze ususe amafilimu aphilayo.

Ukusebenza ngamafutha we-silicone
Uma usebenza ngamafutha we-silicone kunconywa ukuthi ungavumeli inzwa yome. Amafutha e-Silicone angahlanzwa ngokusebenzisa izihlanzi ezikhethekile. Bheka umhlinzeki wakho kawoyela we-silicone ukuze uthole izinto zokuhlanza ezihambisana nengilazi.

Ukusebenza ngamapende noma iglue
Uma usebenza ngamapende noma ama-glues kubalulekile ukuthi ungavumeli inzwa yome. Ngokuvamile, amapende nama-glues awasakwazi ukususwa ngemva kokuba omile. Hlanza inzwa ngezinto zokuhlanza ezinconywe upende wakho noma umenzi weglue ahambisana nengilazi. Qinisekisa ukuthi uthole inqubo yokuhlanza enhle ngaphambi kokwenza izivivinyo zokuqala, futhi uhlale uhlanzekile ngemva nje kokukhipha inzwa.

Ukusebenza ngama-alcohols noma ama-solvents
Ngokungafani nezinye izinto eziwuketshezi, ama-alcohol nama-solvents akubalulekile futhi i-isopropanol (IPA) emfushane yanele ukuhlanza izindonga ze-capillary.

Okunye uketshezi noma izinhlelo zokusebenza
Uma ungaqiniseki ngohlelo lwakho lokusebenza nokuthi ungayihlanza kanjani inzwa yokugeleza, sicela uthinte u-FLUIGENT ukuze uthole ukwesekwa okwengeziwe ku- support@uigent.com.

Izixazululo zokuhlanza ezikhonjiwe

Ukuhlanza Izindlela ezinganconywa
Ngokuvamile, noma yikuphi ukuhlanza ngezindlela zemishini kufanele kugwenywe. Ungalokothi ungene endleleni yenzwa unezinto ezicijile ezingaklwebha ingilazi. Ngaphezu kwalokho, awekho ama-abrasives noma uketshezi oluqukethe izinto eziqinile ezingagaya indawo ehlanzekile okufanele zisetshenziswe. Noma yini ethinta udonga lwengilazi izodala ukuphambuka ekusebenzeni kokulinganisa noma ilimaze unaphakade inzwa. Ama-asidi aqinile nezisekelo akufanele futhi asetshenziselwe ukuhlanza inzwa. Ama-Acid ngezinye izikhathi angasetshenziswa ezindaweni eziphansi kanye namazinga okushisa aphansi. Ngaphambi kokusebenzisa i-asidi hlola ukuthi ihambisana kangakanani nengilazi ye-borosilicate 3.3 (Pyrex® noma iDuran®).

UKUSEBENZA NEWARANTI

Uhlelo lomsebenzi

Imigomo yewaranti

Okuhlanganisa Iwaranti
Lesi siqinisekiso sinikezwa ngabakwa-Fluigent futhi sisebenza kuwo wonke amazwe. Umkhiqizo wakho we-Fluigent uqinisekiswa unyaka owodwa kusukela ngosuku owalethwa ngalo elabhorethri yakho ngokumelene nobuthakathaka bezinto zokwakha nomsebenzi. Uma kutholakala ukuthi kunesici phakathi nesikhathi sewaranti, umkhiqizo wakho we-Fluigent uzokhandwa noma ushintshwe mahhala.

Yini Le Iwaranti Ayikhavi
Lesi siqinisekiso asikubandakanyi ukunakekela okujwayelekile, noma umonakalo odaleka ngenxa yokwehluleka ukugcina umkhiqizo ngokuhambisana nemiyalelo ehlinzekwe ngabakwa-Fluigent. Lesi siqiniseko futhi asibandakanyi umonakalo ovela ngenxa yokusebenzisa kabi noma ukuhlukumeza ngamabomu, ukuguqulwa noma ukwenziwa ngendlela oyifisayo, noma ukulungiswa abantu abangagunyaziwe.

Uyithola Kanjani Isevisi
Uma kukhona okungahambi kahle, xhumana nomthengisi we-Fluigent othenge kuye umkhiqizo wakho. Hlela isikhathi esivumelanayo ukuze omele isevisi ye-Fluigent axoxisane ngenkinga futhi athole isisombululo sokulungisa inkinga. Kuzovunyelwa noma yikuphi ukulungiswa okukude, kodwa uma kwenzeka kudingeka kuthathwe izinyathelo ezengeziwe, isistimu izobuyela emahhovisi e-Fluigent (ngaphandle kwezindleko ezengeziwe, kuphela uma ingaphansi kwewaranti).

Izimo zewaranti yilezi:

• Ungalokothi uvule i-FLOWBOARD kanye namadivayisi we-FLOW UNIT
• Ungasebenzisi ezinye izintambo kunezintambo ezinikezwe i-Fluigent
• Vimbela izinto zangaphandle noma uketshezi ekungeneni ku-FLOWBOARD
• Vimbela izinto zangaphandle ukuthi zingangeni ku-FLOW UNIT
• Ungabeki umkhiqizo endaweni engazinzile, beka iyunithi endaweni enezinga eliphezulu kanye nokwesekwa okuqinile nokuzinzile.
• Hlonipha ukuhambisana kwezinga lokushisa (kusuka ku-5°C ukuya ku-50 °C)
• Hlunga isixazululo sakho, uma kungenzeka wengeze isihlungi kumzila ogelezayo (§ 10) futhi uhlanze I-FLOW UNIT yakho ngemva kokusetshenziswa ngakunye, ikakhulukazi i-FLOW UNIT XS (cf § 4.3). Ububanzi be-FLOW UNIT XS capillary buncane: 25 µm. I-Fluigent yenqaba noma isiphi isibopho esimweni sokuvala noma ukuguqulwa kwendawo.
• Ungavumeli i-FLOW UNIT ukuthi yome nemidiya eshubhuni le-capillary ngaphandle kokucwenga kuhlanzekile kuqala.
• U-Fluigent weluleka ukuthi kuqashwe inqubo yokuhlanza ngemuva kokusetshenziswa.
• Amapulagi aphuzi we-FLOW UNIT kufanele afakwe ukuze agcinwe
• Hlola ukuhambisana koketshezi nezinto ezimanzisiwe ze-FLOW UNIT ngaphambi kokuwusebenzisa noma ucele ukwesekwa kwamakhasimende e-Fluigent.
• Ikhasimende linesibopho soketshezi olusetshenziswa neFLOW UNIT. Ngaphambi kokusetshenziswa, ikhasimende kufanele lihlole ukuhambisana koketshezi oluketshezi ne-FLOW UNIT .

Ngokusetshenziswa okukhethekile, sicela uxhumane nethimba lethu Lokusekela ku support@uigent.com

Amadokhumenti / Izinsiza

FLUIGENT FLOW UNIT Izinzwa Zokugeleza Okubili [pdf] Imaniwali yosebenzisayo I-FLOW UNIT, Izinzwa Zokugeleza Ezikabili, Izinzwa Zokugeleza Okuphindwe kabili kwe-FLOW UNIT

Izithenjwa

• Imaniwali yosebenzisayo