I-BIOSENSORS DC-0 eguqukayo v5.1 Ikhithi Yokulawula Ukuminyana
Izici Eziyinhloko
- Intambo ye-Anchor ehambisanayo 1 futhi i-Anchor strand ehambisanayo 2 ngoba low density isizukulwane of heliX ®
- I-Adapter Chip Spot 1 ne-Spot 2.
- Iyahambisana heliX ® I-adaptha Chip.
- Ilungele HLANGANISA RUN sample ukulungiselela.
- Le kit inika amandla ukulinganisa ukuminyana kwe-ligand endaweni yenzwa.
® I-Adapter Chip Overview
Izindawo ezi-2 ezinokulandelana kwesikhonkwane oku-2 okuhlukile kwekheli elinekhodi ye-DNA.
Incazelo Yomkhiqizo
Inombolo Ye-oda: DC-0
Ithebula 1. Okuqukethwe kanye Nokwaziswa Kwesitoreji
| Okubalulekile | Cap | Ukugxila | Inani | Ibhafa | Isitoreji |
| I-cAnchor strand 1 | Okubomvu | 200 nM | 5 x 200µL | I-TE40 [1] | -20°C |
| I-cAnchor strand 2 | Okubomvu | 200 nM | 5 x 200µL | I-TE40 [1] | -20°C |
Okokusetshenziswa kocwaningo kuphela.
Lo mkhiqizo unomkhawulo wempilo yeshelufu, sicela ubone idethi yokuphelelwa yisikhathi kulebula.
Ukuze ugweme imijikelezo eminingi yokuncibilika kweqhwa sicela uliquot i-nanolayer.
Ukulungiselela | HLANGANISA RUN
I-in-solution hybridization ye-adaptha nemicu ye-ligand:
- Hlanganisa umucu we-Adaptha 1 – Ra (400 nM) kanye ne-conjugated Ligand strand (500 nM) ngesilinganiso esingu-1:1 (v/v).
- Faka isixazululo sesinyathelo 1 ku-RT ku-600 rpm imizuzu engama-30 ukuze uqinisekise ukuhlanganisa okuphelele.
- Hlanganisa isisombululo sesinyathelo sesi-2 ne-Adapter strand 2 – Ra – lfs (200 nM) ngesilinganiso esingu-1:1 (v/v). Isixazululo sesilungele ukusetshenziselwa ukusebenza kwe-biochip Ukuzinza kwesixazululo kuhlobene nokuzinza kwemvukuzane ye-ligand
Ukulungiselela | Ukuhlukahluka kwe-Ligand Density
Ukuguquguquka okulula kwe-ligand density endaweni yenzwa: - Hlanganisa umucu we-cantor 1 ne-Anchor strand 2 ku-1:1 isilinganiso (v/v).
- Hlanganisa isixazululo sesinyathelo sesi-3 nesixazululo sesinyathelo sesi-4 ngesilinganiso sokuminyana kwe-chip oyifunayo, isb:
- a. 1:1 isilinganiso (v/v) ngo-50% we-ligand density
- b. 1:2 isilinganiso (v/v) ngo-30% we-ligand density
- c. 1:9 isilinganiso (v/v) ngo-10% we-ligand density
Isixazululo sesilungele ukusetshenziswa ekusebenzeni kwe-biochip
Example
Ivolumu edingekayo yokusebenza oku-3: 100µL ngomdlalo wamanqamu ukuminyana kwe-ligand of 10 %.
| I-Vial 4 | I-Vial 4 | |
| I-adaptha umucu 1 – Ra with ligand (100 nM) I-adaptha umucu 2 – Ra – lfs (100 nM) | I-cAnchor strand 1 (200 | I-cAnchor strand 2 (200 nM |
| 10µL | 45µ | 45µL |
Hlanganisa i-vial 3 kanye ne-vial 4 ukuze uthole i-100 µL yesisombululo se-DNA esilungele ukusetshenziswa.
Izinombolo Ze-oda Eziwusizo
| Umkhiqizo | Phawula | I-oda No |
| I-heliX ® I-Adapter Chip | I-Chip enezindawo zokutholwa ezingu-2 | ADP-48-2-0 |
| heliX ® Amine Coup | Okokusabela kokuhlangana okukodwa okuhlanu | I-HK-NHS-1 |
| I-adaptha umucu 1 – R | 400 nM | AS-1-Ra |
| I-adaptha umucu 2 - | 200 nM, eyenziwe ngaphambilini nge-Ligand-free stran | AS-2-Ra |
Ukwesekwa Kwamakhasimende
Oxhumana naye
I-Dynamic Biosensors GmbH
I-Perchtinger Str. 8/10
81379 eMunich
EJalimane
Inkampani engu-Dynamic Biosensors, Inc.
300 Trade Center, Suite 1400
I-Woburn, i-MA 01801
USA
Oda Ulwazi order@dynamic-biosensors.com
Ukusekela kwezobuchwepheshe support@dynamic-biosensors.com
www.dynamic-biosensors.com
Izinsimbi nama-chips akhiwe futhi akhiqizwa eJalimane.
©2024 Dynamic Biosensors GmbH | Dynamic Biosensors, Inc. Wonke amalungelo agodliwe.
[1] TE40: 10 mM Tris, 40 mM NaCl, 0.05 % Tween20, 50 μM EDTA, 50 μM EGTA
www.dynamic-biosensors.co
Amadokhumenti / Izinsiza
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I-BIOSENSORS DC-0 eguqukayo v5.1 Ikhithi Yokulawula Ukuminyana [pdf] Imaniwali yosebenzisayo DC-0 v5.1 Density Control Kit, DC-0 v5.1, Density Control Kit, Control Kit, Kit |